module neb e7546 blunt ta ligase master mix neb m0367 nebnext quick ligation module neb e6056s native barcoding kit 24 v14  (New England Biolabs)

Journal: Cell chemical biology

Article Title: Linking Genomic and Metabolomic Natural Variation Uncovers Nematode Pheromone Biosynthesis

doi: 10.1016/j.chembiol.2018.04.004

Figure Lengend Snippet:

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat Anti 6 - His Polyclonal Antibody, HRP Conjugate QED Bioscience Cat# 18812P Bacterial and Virus Strains E. coli OP50 Lab of Leon Avery N/A E. coli HB101 Lab of Leon Avery N/A NEB ® 5 - alpha Competent E. coli (High Efficiency) New England Biolabs Cat# C2987H BL21(DE3) Competent E. coli New England Biolabs Cat# C2527H Tuner™(DE3) Competent E. coli Novagen Cat# 70623 - 3 Chemicals, Peptides, and Recombinant Proteins SMARTer ® RACE 5′/3′ Kit Clontech, Mountain View, USA Cat#634859 Master™ PureDNA extraction kit Epicentre Cat#MCD85201 Direct-zolTM RNA MiniPrep Zymo Research Germany Cat# R2070 QIAquick PCR Purification Kit Qiagen, Germany Cat# 28104 Illumina’s paired-end protocol Illumina, Netherlands Cat#PE-102-1001 Herculase II fusion DNA Polymerase kit Agilent, Germany Cat#600675 Nanopore Sequencing Kit protocol SQK-MAP005 (Version MN005_1115_revE_26Nov2014) Oxford Nanopore Technologies Cat# SQK-LWP001 NEBNext ® End Repair Module NEB Cat# E6050 Blunt/TA Ligase Master Mix NEB Cat# M0367 Dynabeads ® His-Tag Isolation and Pulldown Thermo Fisher Scientific Cat#10103D TRIzol™ Reagent Invitrogen Cat# 15596018 Critical Commercial Assays K. lactis Protein Expression Kit New England Biolabs Cat# E1000S Experimental Models: Organisms/Strains C. elegans : COP93 [unc-119(ed3); ttTi5605] Knudra Transgenics N/A C. elegans : N2 Lab of Leon Avery N/A C. elegans : COP1610-1613: knuSi763 [ pnu1497 ( hsp16.41 :: Ppa-uar-1 :: tbb-2utr , unc-119 (+)) ] II ; unc-119 (ed3) III.

Techniques: Recombinant, Purification, Nanopore Sequencing, Isolation, Expressing, Plasmid Preparation, Software